Senthil Kumar Subramanian
Bharathidasan University, India
Title: Investigation of antimicrobial activities and mechanism of silver nanoparticles synthesized from aerial roots of Rhaphidophora aurea (Linden ex Andre) extract
Biography
Biography: Senthil Kumar Subramanian
Abstract
Silver nanoparticles were successfully prepared by eco-friendly and cost effective aerial roots of Rhaphidophora aurea (Linden ex Andre) extract which act as a reducing agent by green synthesis method. The visual color change from colorless to yellow to brown was helpful in confirmation of AgNPs formation. UV Visible (UV-vis) spectroscopy was used to follow the gradual formation of NPs and power X-ray diffraction (XRD) confirmed the face centre cubic crystal structures in software in JANA2006, Edit+3. XRD studies revealed a gradual crystal-lite size reduction at different molar ratios of extract silver salt. The crystallite size was calcu-lated using Debye-Scherer’s formula and found to be in the range 30 nm. Morphology and characterization of AgNPs was done by using FESEM, HRTEM, FTIR and EDAX techniques. FESEM and HRTEM analysis showed that the morphology of the obtained AgNPs was spherical shaped which can be used in variety of biomedical and pharmaceutical applications. The antibacterial actives and mechanism of silver nanoparticles are examined using Escherichia coli, Streptococcus mutants, Bacillus subtilis and Pseudomonas aeruginosa respectively by analyzing the growth permeability and morphology of the bacterial cells following with AgNPs.