Muhammad Ismail
Kohat University of Science & Technology, Pakistan
Title: Green synthesis of silver and copper nanoparticles and their biological evaluation
Biography
Biography: Muhammad Ismail
Abstract
Silver and copper nanoparticles are known to have inhibitory, bactericidal effects and catalytic degradation effect on methyl red dye. Several methods about synthesis of nanoparticles have been reported including chemical and physical methods which are associated with environmental concerns. In present study, here, we report biosynthesis of silver and copper nanoparticles using Duranta erecta fruits extract at ambient temperature and pressure. Biosynthesis of nanoparticles is cheap, low cost, non-toxic, high-yield and eco-friendly method. Size and reduction time of Ag and Cu NPs were compared with chemically synthesized hydrazine mediated Ag and Cu NPs. Nanoparticles formation was confirmed by UV/visible spectrophotometry due to the surface plasmon resonance, in which silver show at 447 nm while copper at 597 nm. Percent reduction of metal salts was determined using atomic adsorption spectrometry. The size, shape and morphology of the synthesized nanoparticles were examined using powder XRD and FE-SEM respectively. EDX analysis was performed showing strong signal peak for elemental silver and copper also confirm the formation of nanoparticles. The biosynthesized silver nanoparticles show excellent antibacterial activity and synergistic effect with antibiotic gemifloxacin against human pathogen gram positive, Staphylococcus aureus and gram negative, Escherichia coli as compared to copper nanoparticles. The prepared nanoparticles exhibit excellent methyl red photocatalytic degradation activity proved by decrease in absorbance with time. Besides these, the biologically synthesized silver and copper nanoparticles also show excellent 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity.